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time-lapse fluorescence laser confocal microscopy  (KEYENCE)

 
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    KEYENCE time-lapse fluorescence laser confocal microscopy
    (A) Day 50–60 organoids from human embryonic stem cells (hESCs) (SEES1 cells) immunostained with markers for intestinal differentiation: villin, leucine-rich repeat containing G protein–coupled receptor 5 (LGR5), CDX2, E-cadherin (ECAD), chromogranin A (CGA), mucin-2 (MUC2), defensin α-6, Paneth cell–specific (DEFA6), α-smooth muscle actin (SMA), and protein gene product 9.5 (PGP9.5). Cell nuclei were counterstained with DAPI. PGP9.5-positive enteric neuronal cells within the α-SMA–positive myenteric area are indicated by yellow arrowheads. Scale bar: 50 μm (VILLIN, LGR5, SMA); 100 μm (ECAD and MUC2). (B) An enterocyte with a characteristic brush border (left), and Paneth cells with secretory granules (black arrowhead) and goblet cells containing mucin granules (yellow arrowhead) (right). Scale bar: 10 μm (left); 5 μm (right). (C) Organoids that developed from SEES1 cells expressed EGFP under the LGR5 promoter (green), indicating that they were LGR5-positive gut organoids. Gut tube-like architecture in a day 34 organoid (red square; high-magnification view, white square) (top row). A small number of EGFP-positive cells were detected in the day 34 organoid (white square) by <t>fluorescence</t> <t>microscopy</t> (bottom left). The number of EGFP-positive cells increased at day 41 (bottom right). Scale bar: 300 μm (top left), 100 μm (top right and bottom row).
    Time Lapse Fluorescence Laser Confocal Microscopy, supplied by KEYENCE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/time-lapse+fluorescence+laser+confocal+microscopy/pmc05214546-364-11-16?v=KEYENCE
    Average 90 stars, based on 1 article reviews
    time-lapse fluorescence laser confocal microscopy - by Bioz Stars, 2026-08
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    Images

    1) Product Images from "A xenogeneic-free system generating functional human gut organoids from pluripotent stem cells"

    Article Title: A xenogeneic-free system generating functional human gut organoids from pluripotent stem cells

    Journal: JCI Insight

    doi: 10.1172/jci.insight.86492

    (A) Day 50–60 organoids from human embryonic stem cells (hESCs) (SEES1 cells) immunostained with markers for intestinal differentiation: villin, leucine-rich repeat containing G protein–coupled receptor 5 (LGR5), CDX2, E-cadherin (ECAD), chromogranin A (CGA), mucin-2 (MUC2), defensin α-6, Paneth cell–specific (DEFA6), α-smooth muscle actin (SMA), and protein gene product 9.5 (PGP9.5). Cell nuclei were counterstained with DAPI. PGP9.5-positive enteric neuronal cells within the α-SMA–positive myenteric area are indicated by yellow arrowheads. Scale bar: 50 μm (VILLIN, LGR5, SMA); 100 μm (ECAD and MUC2). (B) An enterocyte with a characteristic brush border (left), and Paneth cells with secretory granules (black arrowhead) and goblet cells containing mucin granules (yellow arrowhead) (right). Scale bar: 10 μm (left); 5 μm (right). (C) Organoids that developed from SEES1 cells expressed EGFP under the LGR5 promoter (green), indicating that they were LGR5-positive gut organoids. Gut tube-like architecture in a day 34 organoid (red square; high-magnification view, white square) (top row). A small number of EGFP-positive cells were detected in the day 34 organoid (white square) by fluorescence microscopy (bottom left). The number of EGFP-positive cells increased at day 41 (bottom right). Scale bar: 300 μm (top left), 100 μm (top right and bottom row).
    Figure Legend Snippet: (A) Day 50–60 organoids from human embryonic stem cells (hESCs) (SEES1 cells) immunostained with markers for intestinal differentiation: villin, leucine-rich repeat containing G protein–coupled receptor 5 (LGR5), CDX2, E-cadherin (ECAD), chromogranin A (CGA), mucin-2 (MUC2), defensin α-6, Paneth cell–specific (DEFA6), α-smooth muscle actin (SMA), and protein gene product 9.5 (PGP9.5). Cell nuclei were counterstained with DAPI. PGP9.5-positive enteric neuronal cells within the α-SMA–positive myenteric area are indicated by yellow arrowheads. Scale bar: 50 μm (VILLIN, LGR5, SMA); 100 μm (ECAD and MUC2). (B) An enterocyte with a characteristic brush border (left), and Paneth cells with secretory granules (black arrowhead) and goblet cells containing mucin granules (yellow arrowhead) (right). Scale bar: 10 μm (left); 5 μm (right). (C) Organoids that developed from SEES1 cells expressed EGFP under the LGR5 promoter (green), indicating that they were LGR5-positive gut organoids. Gut tube-like architecture in a day 34 organoid (red square; high-magnification view, white square) (top row). A small number of EGFP-positive cells were detected in the day 34 organoid (white square) by fluorescence microscopy (bottom left). The number of EGFP-positive cells increased at day 41 (bottom right). Scale bar: 300 μm (top left), 100 μm (top right and bottom row).

    Techniques Used: Fluorescence, Microscopy

    (A) Day 115 gut organoids from hESCs (SEES1 cells) immunostained with markers for the cystic fibrosis conductance regulator (CFTR). CFTR was present in the epithelial layers of the gut organoids as well as in the control human intestine. CFTR (green), actin (red), and DAPI (blue). Scale bar: 100 μm. (B) Quantification of forskolin-induced gut organoid swelling. Gut organoids (green) derived from SEES1 cells were monitored by time-lapse fluorescence laser confocal microscopy (Keyence). The organoid surface area (red) was quantified using Hybrid Cell Count BZ-H3C (Keyence). The normalized total organoid surface area was calculated and averaged from 3 individual wells per treatment (n = 3 for each group). A forskolin-induced organoid (19 minutes) was overlaid with a pretreated organoid (0 minutes; 569 μm).
    Figure Legend Snippet: (A) Day 115 gut organoids from hESCs (SEES1 cells) immunostained with markers for the cystic fibrosis conductance regulator (CFTR). CFTR was present in the epithelial layers of the gut organoids as well as in the control human intestine. CFTR (green), actin (red), and DAPI (blue). Scale bar: 100 μm. (B) Quantification of forskolin-induced gut organoid swelling. Gut organoids (green) derived from SEES1 cells were monitored by time-lapse fluorescence laser confocal microscopy (Keyence). The organoid surface area (red) was quantified using Hybrid Cell Count BZ-H3C (Keyence). The normalized total organoid surface area was calculated and averaged from 3 individual wells per treatment (n = 3 for each group). A forskolin-induced organoid (19 minutes) was overlaid with a pretreated organoid (0 minutes; 569 μm).

    Techniques Used: Control, Derivative Assay, Fluorescence, Confocal Microscopy, Cell Counting



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    (A) Day 50–60 organoids from human embryonic stem cells (hESCs) (SEES1 cells) immunostained with markers for intestinal differentiation: villin, leucine-rich repeat containing G protein–coupled receptor 5 (LGR5), CDX2, E-cadherin (ECAD), chromogranin A (CGA), mucin-2 (MUC2), defensin α-6, Paneth cell–specific (DEFA6), α-smooth muscle actin (SMA), and protein gene product 9.5 (PGP9.5). Cell nuclei were counterstained with DAPI. PGP9.5-positive enteric neuronal cells within the α-SMA–positive myenteric area are indicated by yellow arrowheads. Scale bar: 50 μm (VILLIN, LGR5, SMA); 100 μm (ECAD and MUC2). (B) An enterocyte with a characteristic brush border (left), and Paneth cells with secretory granules (black arrowhead) and goblet cells containing mucin granules (yellow arrowhead) (right). Scale bar: 10 μm (left); 5 μm (right). (C) Organoids that developed from SEES1 cells expressed EGFP under the LGR5 promoter (green), indicating that they were LGR5-positive gut organoids. Gut tube-like architecture in a day 34 organoid (red square; high-magnification view, white square) (top row). A small number of EGFP-positive cells were detected in the day 34 organoid (white square) by <t>fluorescence</t> <t>microscopy</t> (bottom left). The number of EGFP-positive cells increased at day 41 (bottom right). Scale bar: 300 μm (top left), 100 μm (top right and bottom row).
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    (A) Day 50–60 organoids from human embryonic stem cells (hESCs) (SEES1 cells) immunostained with markers for intestinal differentiation: villin, leucine-rich repeat containing G protein–coupled receptor 5 (LGR5), CDX2, E-cadherin (ECAD), chromogranin A (CGA), mucin-2 (MUC2), defensin α-6, Paneth cell–specific (DEFA6), α-smooth muscle actin (SMA), and protein gene product 9.5 (PGP9.5). Cell nuclei were counterstained with DAPI. PGP9.5-positive enteric neuronal cells within the α-SMA–positive myenteric area are indicated by yellow arrowheads. Scale bar: 50 μm (VILLIN, LGR5, SMA); 100 μm (ECAD and MUC2). (B) An enterocyte with a characteristic brush border (left), and Paneth cells with secretory granules (black arrowhead) and goblet cells containing mucin granules (yellow arrowhead) (right). Scale bar: 10 μm (left); 5 μm (right). (C) Organoids that developed from SEES1 cells expressed EGFP under the LGR5 promoter (green), indicating that they were LGR5-positive gut organoids. Gut tube-like architecture in a day 34 organoid (red square; high-magnification view, white square) (top row). A small number of EGFP-positive cells were detected in the day 34 organoid (white square) by <t>fluorescence</t> <t>microscopy</t> (bottom left). The number of EGFP-positive cells increased at day 41 (bottom right). Scale bar: 300 μm (top left), 100 μm (top right and bottom row).
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    Image Search Results


    (A) Day 50–60 organoids from human embryonic stem cells (hESCs) (SEES1 cells) immunostained with markers for intestinal differentiation: villin, leucine-rich repeat containing G protein–coupled receptor 5 (LGR5), CDX2, E-cadherin (ECAD), chromogranin A (CGA), mucin-2 (MUC2), defensin α-6, Paneth cell–specific (DEFA6), α-smooth muscle actin (SMA), and protein gene product 9.5 (PGP9.5). Cell nuclei were counterstained with DAPI. PGP9.5-positive enteric neuronal cells within the α-SMA–positive myenteric area are indicated by yellow arrowheads. Scale bar: 50 μm (VILLIN, LGR5, SMA); 100 μm (ECAD and MUC2). (B) An enterocyte with a characteristic brush border (left), and Paneth cells with secretory granules (black arrowhead) and goblet cells containing mucin granules (yellow arrowhead) (right). Scale bar: 10 μm (left); 5 μm (right). (C) Organoids that developed from SEES1 cells expressed EGFP under the LGR5 promoter (green), indicating that they were LGR5-positive gut organoids. Gut tube-like architecture in a day 34 organoid (red square; high-magnification view, white square) (top row). A small number of EGFP-positive cells were detected in the day 34 organoid (white square) by fluorescence microscopy (bottom left). The number of EGFP-positive cells increased at day 41 (bottom right). Scale bar: 300 μm (top left), 100 μm (top right and bottom row).

    Journal: JCI Insight

    Article Title: A xenogeneic-free system generating functional human gut organoids from pluripotent stem cells

    doi: 10.1172/jci.insight.86492

    Figure Lengend Snippet: (A) Day 50–60 organoids from human embryonic stem cells (hESCs) (SEES1 cells) immunostained with markers for intestinal differentiation: villin, leucine-rich repeat containing G protein–coupled receptor 5 (LGR5), CDX2, E-cadherin (ECAD), chromogranin A (CGA), mucin-2 (MUC2), defensin α-6, Paneth cell–specific (DEFA6), α-smooth muscle actin (SMA), and protein gene product 9.5 (PGP9.5). Cell nuclei were counterstained with DAPI. PGP9.5-positive enteric neuronal cells within the α-SMA–positive myenteric area are indicated by yellow arrowheads. Scale bar: 50 μm (VILLIN, LGR5, SMA); 100 μm (ECAD and MUC2). (B) An enterocyte with a characteristic brush border (left), and Paneth cells with secretory granules (black arrowhead) and goblet cells containing mucin granules (yellow arrowhead) (right). Scale bar: 10 μm (left); 5 μm (right). (C) Organoids that developed from SEES1 cells expressed EGFP under the LGR5 promoter (green), indicating that they were LGR5-positive gut organoids. Gut tube-like architecture in a day 34 organoid (red square; high-magnification view, white square) (top row). A small number of EGFP-positive cells were detected in the day 34 organoid (white square) by fluorescence microscopy (bottom left). The number of EGFP-positive cells increased at day 41 (bottom right). Scale bar: 300 μm (top left), 100 μm (top right and bottom row).

    Article Snippet: Five μM forskolin was added, and organoid morphology was monitored by time-lapse fluorescence laser confocal microscopy (Keyence).

    Techniques: Fluorescence, Microscopy

    (A) Day 115 gut organoids from hESCs (SEES1 cells) immunostained with markers for the cystic fibrosis conductance regulator (CFTR). CFTR was present in the epithelial layers of the gut organoids as well as in the control human intestine. CFTR (green), actin (red), and DAPI (blue). Scale bar: 100 μm. (B) Quantification of forskolin-induced gut organoid swelling. Gut organoids (green) derived from SEES1 cells were monitored by time-lapse fluorescence laser confocal microscopy (Keyence). The organoid surface area (red) was quantified using Hybrid Cell Count BZ-H3C (Keyence). The normalized total organoid surface area was calculated and averaged from 3 individual wells per treatment (n = 3 for each group). A forskolin-induced organoid (19 minutes) was overlaid with a pretreated organoid (0 minutes; 569 μm).

    Journal: JCI Insight

    Article Title: A xenogeneic-free system generating functional human gut organoids from pluripotent stem cells

    doi: 10.1172/jci.insight.86492

    Figure Lengend Snippet: (A) Day 115 gut organoids from hESCs (SEES1 cells) immunostained with markers for the cystic fibrosis conductance regulator (CFTR). CFTR was present in the epithelial layers of the gut organoids as well as in the control human intestine. CFTR (green), actin (red), and DAPI (blue). Scale bar: 100 μm. (B) Quantification of forskolin-induced gut organoid swelling. Gut organoids (green) derived from SEES1 cells were monitored by time-lapse fluorescence laser confocal microscopy (Keyence). The organoid surface area (red) was quantified using Hybrid Cell Count BZ-H3C (Keyence). The normalized total organoid surface area was calculated and averaged from 3 individual wells per treatment (n = 3 for each group). A forskolin-induced organoid (19 minutes) was overlaid with a pretreated organoid (0 minutes; 569 μm).

    Article Snippet: Five μM forskolin was added, and organoid morphology was monitored by time-lapse fluorescence laser confocal microscopy (Keyence).

    Techniques: Control, Derivative Assay, Fluorescence, Confocal Microscopy, Cell Counting